human hepatocyte carcinoma hepg2 cells  (ATCC)

Journal: Biotechnology Reports

Article Title: Insulin Resistance, Anti-inflammatory, and Antioxidant In vitro Activity of Heterologously Expressed Arenin from Dryophytes arenicolor

doi: 10.1016/j.btre.2026.e00947

Figure Lengend Snippet: In vitro modulation of hepatic insulin resistance by arenin in hepatic insulin-resistant (HepG2) cells . Arenin concentrations (3.90–1000 µg/mL) were incubated for 5 h in hepatic insulin-resistant cells. Negative control (N) cells were cultured in the absence of insulin, whereas insulin-resistant (IR) control cells were cultured in the presence of insulin. Rosiglitazone (ROSI) at 10 μM (3.57 µg/mL) was used as the positive reference control. Different letters abc indicate statistical differences between concentrations analyzed using Tukey test ( p < 0.05).

Article Snippet: Human hepatocyte carcinoma (HepG2) cells, human primary dermal fibroblasts (HDFa) cells, and murine macrophage (RAW 264.7) cells were obtained from the American Type Culture Collection (ATCC, VA, USA).

Techniques: In Vitro, Incubation, Negative Control, Cell Culture, Control

Journal: Chemmedchem

Article Title: Structure‐Based Discovery of Obeticholic Acid Derivatives as Novel Farnesoid X Receptor Partial Agonists with Improved Selectivity and Reduced Off‐Target Effects

doi: 10.1002/cmdc.202500960

Figure Lengend Snippet: Results of RT‐PCR in HepG2 cell line following overnight treatment with endogenous FXR agonist CDCA ( red ) (30 μM), OCA ( purple ) (10 µM), compound 2 ( green ) (10 μM and 30 μM), or compound 16 ( blue ) (10 μM and 30 μM). Not treated cells ( white ) were stimulated with DMSO 0.1. % Statistical significance was analyzed by One‐way Anova‐GraphPad Prism 5. ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001 vs 0.1% NT = not treated (DMSO).

Article Snippet: HepG2 human hepatocellular carcinoma cells (RRID:CVCL_0027, HB‐8065, ATCC) were cultured in E‐MEM medium supplemented with 1% penicillin/streptomycin, 1% L‐glutamine, and 10% fetal bovine serum (Life Technologies).

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: Biomaterials

Article Title: A novel Kupffer cell-targeting nanoparticle system to Mitigate alcohol-associated liver disease

doi: 10.1016/j.biomaterials.2025.123623

Figure Lengend Snippet: Release of DEX from NPs in 1X PBS for 21 days at 37°C and two different pH: 6.0 and 7.4; (a) PC-0.2+DEX; (b) PC-0.5+DEX; (c) PC-1+DEX: only PC-1+DEX showed significantly high pH-dependent release of DEX; n = 3 (d) cytocompatibility studies in (i) HepG2 cells and (ii) DTHP1 (macrophages) confirmed cytocompatibility of PC-1 and PC-1+INT NPs in both cell lines 24 h after treatment; n = 4.

Article Snippet: Human hepatocellular carcinoma cell line HepG2 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Biomaterials

Article Title: A novel Kupffer cell-targeting nanoparticle system to Mitigate alcohol-associated liver disease

doi: 10.1016/j.biomaterials.2025.123623

Figure Lengend Snippet: Uptake of NPs by DTHP1 and HepG2 cells treated with 1 μg/ml LPS and 0.16 % (v/v) EtOH; (a) quantitative analysis of NP uptake at different concentrations, confirmed higher, dose-dependent uptake by DTHP1 cells.; n = 4 (b) fluorescence imaging of NP uptake also confirmed higher uptake in DTHP1 than in HepG2 cells: green-coumarin-6 loaded NPs, blue-nuclei (scale bar = 150 μm) (c) quantification of NP uptake after cells were pre-treated with free INT (0.1 μM and 1 μM); uptake of the PC-1+INT NPs reduced due to the free INT pretreatment, confirming Gpbar1 targeting capabilities of the NPs.

Article Snippet: Human hepatocellular carcinoma cell line HepG2 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Fluorescence, Imaging